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bbs i digested vector pspcas9 bb 2a gfp  (Addgene inc)


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    Structured Review

    Addgene inc bbs i digested vector pspcas9 bb 2a gfp
    Bbs I Digested Vector Pspcas9 Bb 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bbs+i+digested+vector+pspcas9+bb+2a+gfp/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__03__26__714469-179-21-28
    Average 96 stars, based on 4086 article reviews
    bbs i digested vector pspcas9 bb 2a gfp - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Multiple Porcine Innate Immune Signaling Pathways Are Involved in the Anti-PEDV Response
    Article Snippet: For each gene, 2–3 gRNAs were selected , and the annealed gRNA encoding DNA pairs were ligated with the Bbs I digested vector pSpCas9(BB)-2A-GFP (pX458, Addgene, Watertown, NY, USA).

    Article Title: The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function
    Article Snippet: The annealed gRNA encoding DNA pairs were ligated with the Bbs I digested vector pSpCas9(BB)-2A-GFP (pX458, Addgene, Watertown, NY, USA).

    Plasmid Preparation:

    Article Title: ASFV early protein p30 suppresses antiviral type I IFN induction by targeting TRIM21 and RIG-I like receptor signaling adaptor MAVS
    Article Snippet: .. Two pairs of high score gRNAs were selected and the annealed gRNAs encoding DNA (Supplementary Table 4) were ligated with the Bbs I digested vector pSpCas9(BB)-2A GFP (pX458, Addgene). .. Subsequently, each gRNA expressing recombinant pX458 was transfected into MA104 cells using Lipofectamine 2000, and GFP positive cells were sorted from the transfected cells using a BD FACSARia III sorting machine.



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